Title | Non-fouling polymeric surface modification and signal amplification method for biomolecular detection |
Publication Type | Patent |
Year of Publication | 2021 |
Authors | Chilkoti, A |
Date Published | 09/28/2021 |
Assignee | Duke University |
Patent Number | 11130989 |
Abstract | An article such as a biosensor having a nonfouling surface thereon is described. The article comprises: (a) a substrate having a surface portion; (b) a linking layer on the surface portion; (c) a polymer layer comprising brush molecules formed on the linking layer; and (d) optionally but preferably, a first member of a specific binding pair (e.g., a protein, peptide, antibody, nucleic acid, etc.) coupled to the brush molecules. The polymer layer is preferably formed by the process of surface-initiated polymerization (SIP) of monomeric units thereon. Preferably, each of the monomeric units comprises a monomer (for example, a vinyl monomer) core group having at least one protein-resistant head group coupled thereto, to thereby form the brush molecule on the surface portion. Methods of using the articles are also described. |