Simple Fabrication of Antibody Microarrays on Nonfouling Polymer Brushes with Femtomolar Sensitivity for Protein Analytes in Serum and Blood

TitleSimple Fabrication of Antibody Microarrays on Nonfouling Polymer Brushes with Femtomolar Sensitivity for Protein Analytes in Serum and Blood
Publication TypeJournal Article
Year of Publication2009
AuthorsHucknall, A, Kim, D-H, Rangarajan, S, Hill, RT, Reichert, WM, Chilkoti, A
JournalAdvanced Materials
Volume21
Issue19
Pagination1968 - 1971
Date Published05/2009
ISSN15214095
Abstract

A multianalyte antibody array that is spotted on a poly(oligo(ethylene glycol) methacrylate) brush 100 nm thick, grown on glass via surface-initiated atom transfer radical polymerization, has femtomolar limit-of-detection (LOD) of cytokines in serum and whole blood, and a dynamic range of six orders of magnitude for a range of protein analytes.

DOI10.1002/adma.200803125
Short TitleAdv. Mater.