Title | Three-in-One Chromatography-Free Purification, Tag Removal, and Site-Specific Modification of Recombinant Fusion Proteins Using Sortase A and Elastin-like Polypeptides |
Publication Type | Journal Article |
Year of Publication | 2013 |
Authors | Bellucci, JJ, Amiram, M, Bhattacharyya, J, McCafferty, D, Chilkoti, A |
Journal | Angewandte Chemie International Edition |
Volume | 52 |
Issue | 13 |
Pagination | 3703–3708 |
Date Published | 02/2013 |
Abstract | Sorting it out: Applied in tandem, elastin-like polypeptides (ELPs, see scheme) and the Sortase A (SrtA) transpeptidase provide a method for chromatography-free purification of recombinant proteins and optional, site-specific conjugation of the protein to a small molecule (yellow). This system provides an efficient mechanism for generating bioactive proteins at high yields and purities. |
DOI | 10.1002/anie.201208292 |
Short Title | Angew. Chem. Int. Ed. |