Three-in-One Chromatography-Free Purification, Tag Removal, and Site-Specific Modification of Recombinant Fusion Proteins Using Sortase A and Elastin-like Polypeptides

TitleThree-in-One Chromatography-Free Purification, Tag Removal, and Site-Specific Modification of Recombinant Fusion Proteins Using Sortase A and Elastin-like Polypeptides
Publication TypeJournal Article
Year of Publication2013
AuthorsBellucci, JJ, Amiram, M, Bhattacharyya, J, McCafferty, D, Chilkoti, A
JournalAngewandte Chemie International Edition
Volume52
Issue13
Pagination3703–3708
Date Published02/2013
Abstract

Sorting it out: Applied in tandem, elastin-like polypeptides (ELPs, see scheme) and the Sortase A (SrtA) transpeptidase provide a method for chromatography-free purification of recombinant proteins and optional, site-specific conjugation of the protein to a small molecule (yellow). This system provides an efficient mechanism for generating bioactive proteins at high yields and purities.

DOI10.1002/anie.201208292
Short TitleAngew. Chem. Int. Ed.