Protein Microarrays on "Nonfouling" Polymer Brush

We have developed a new protein microarray assay that is built on a non-fouling polymer brush of poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA) that is grown form a surface by surface-initiated polymerization. These “zero background” protein microarrays were successfully used to quantify protein analytes in serum with a femtomolar limit-of-detection (LOD) and a dynamic range of six orders of magnitude of analyte concentration directly from undiluted, whole blood. We have recently extended this approach to create a true point-of-care diagnostic –in which all reagents are printed and stored on the POEGMA brush– called the D4 assay.


Inkjet-printed point-of-care immunoassay on a nanoscale polymer brush enables subpicomolar detection of analytes in blood. D.Y. Joh; A.M. Hucknall; Q. Wei; K.A. Mason; M.L. Lund; C.M. Fontes; R.T. Hill; R. Blair; Z. Zimmers; R.K. Achar; D. Tseng; R. Gordan; M. Freemark; A. Ozcan; A. Chilkoti. (2017).
Poly(oligo(ethylene glycol) methyl ether methacrylate) brushes on high-κ metal oxide dielectric surfaces for bioelectrical environments. D.Youngjin Joh; F. McGuire; R. Abedini-Nassab; J.B. Andrews; R.K. Achar; Z. Zimmers; D. Mozhdehi; R. Blair; F. Albarghouthi; W. Oles; J. Richter; C.M. Fontes; A.M. Hucknall; B.B. Yellen; A.D. Franklin; A. Chilkoti. (2017).
Crystallization kinetics of binary colloidal monolayers. A.T. Pham; R. Seto; J. önke; D.Y. Joh; A. Chilkoti; E. Fried; B.B. Yellen. (2016).
In Pursuit of Zero: Polymer Brushes that Resist the Adsorption of Proteins. A. Hucknall; S. Rangarajan; A. Chilkoti. (2009).
Versatile synthesis and micropatterning of nonfouling polymer brushes on the wafer scale. A. Hucknall; A.J. Simnick; R.T. Hill; A. Chilkoti; A. Garcia; M.S. Johannes; R.L. Clark; S. Zauscher; B.D. Ratner. (2009).