Elastin Fusion Proteins

In this project, we seek to greatly expand upon our ability to modulate the solution behavior of ELP fusion proteins by developing a new generation of ELP fusion proteins that will self-assemble into nanoparticles of defined size and shape or that will exhibit soluble-insoluble phase transition behavior upon binding to another molecule. These ELP fusion proteins will provide new molecular building blocks that will enable new approaches for targeted drug delivery, regenerative medicine and bioanalytical assays.

Publications

Encoding Structure in Intrinsically Disordered Protein Biomaterials. R.L. Strader; Y. Shmidov; A. Chilkoti. (2024).
Programmable synthetic biomolecular condensates for cellular control. Y. Dai; M. Farag; D. Lee; X. Zeng; K. Kim; Hin Son; X. Guo; J. Su; N. Peterson; J. Mohammed; M. Ney; D.Mark Shapiro; R.V. Pappu; A. Chilkoti; L. You. (2023).
IsoTag™AAV: an innovative, scalable & non-chromatographic method for streamlined AAV manufacturing. J. Haley; J.B. Jones; S. Petraki; M. Callander; S. Shrestha; E. Springfield; L. Adamson; A. Chilkoti; M.J. Dzuricky; K.M. Luginbuhl. (2022).
Brachytherapy via a depot of biopolymer-bound 131I synergizes with nanoparticle paclitaxel in therapy-resistant pancreatic tumours. J.L. Schaal; J. Bhattacharyya; J. Brownstein; K.C. Strickland; G. Kelly; S. Saha; J. Milligan; S. Banskota; X. Li; W. Liu; D.G. Kirsch; M.R. Zalutsky; A. Chilkoti. (2022).
Dual agonist fusion proteins. A. Chilkoti; C. Gilroy. (2021).
Microphase Separation of Resilin-like and Elastin-like Diblock Copolypeptides in Concentrated Solutions. L.A. Navarro; J.J. Ryan; M. Dzuricky; M. Gradzielski; A. Chilkoti; S. Zauscher. (2021).
Photo-Crosslinkable Unnatural Amino Acids Enable Facile Synthesis of Thermoresponsive Nano- to Microgels of Intrinsically Disordered Polypeptides. S.A. Costa; J.R. Simon; M. Amiram; L. Tang; S. Zauscher; E.M. Brustad; F.J. Isaacs; A. Chilkoti. (2017).
Micellar self-assembly of recombinant resilin-like/elastin-like block copolypeptides. I. Weitzhandler; M. Dzuricky; I. Hoffmann; F.Garcia Quiroz; M. Gradzielski; A. Chilkoti. (2017).
Predicting Transition Temperatures of Elastin-Like Polypeptide Fusion Proteins. T. Christensen; W. Hassouneh; K.A. Trabbic-Carlson; A. Chilkoti. (2013).
Unexpected Multivalent Display of Proteins by Temperature Triggered Self-Assembly of Elastin-like Polypeptide Block Copolymers. W. Hassouneh; K. Fischer; S.R. MacEwan; R. Branscheid; C.L. Fu; R. Liu; M. Schmidt; A. Chilkoti. (2012).
Chapter nine - Fusions of Elastin-Like Polypeptides to Pharmaceutical Proteins. W. Hassouneh; S.R. MacEwan; A. Chilkoti. (2012).
Fusion order controls expression level and activity of elastin-like polypeptide fusion proteins. T. Christensen; M. Amiram; S. Dagher; K.A. Trabbic-Carlson; M.F. Shamji; L.A. Setton; A. Chilkoti. (2009).