We have developed a new protein microarray assay that is built on a non-fouling polymer brush of poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA) that is grown form a surface by surface-initiated polymerization. These “zero background” protein microarrays were successfully used to quantify protein analytes in serum with a femtomolar limit-of-detection (LOD) and a dynamic range of six orders of magnitude of analyte concentration directly from undiluted, whole blood. We have recently extended this approach to create a true point-of-care diagnostic –in which all reagents are printed and stored on the POEGMA brush– called the D4 assay.
Protein Microarrays on "Nonfouling" Polymer Brush
Publications
Systems and devices for protease detection based on engineered polymers and biopolymers and methods of use. G.P. Lopez; A. Chilkoti; A. Ghoorchian; F.Garcia Quiroz. (2022).
Nonfouling biosensors. A. Chilkoti; A. Franklin; B. Yellen; A. Hucknall; D. Joh; R. Abedini-Nassab; J. Andrews. (2022).
Plasmonic Fluorescence Enhancement in Diagnostics for Clinical Tests at Point‐of‐Care: A Review of Recent Technologies. D. Semeniak; D.F. Cruz; A. Chilkoti; M.H. Mikkelsen. (2022).
Inkjet-printed point-of-care immunoassay on a nanoscale polymer brush enables subpicomolar detection of analytes in blood. D.Y. Joh; A.M. Hucknall; Q. Wei; K.A. Mason; M.L. Lund; C.M. Fontes; R.T. Hill; R. Blair; Z. Zimmers; R.K. Achar; D. Tseng; R. Gordan; M. Freemark; A. Ozcan; A. Chilkoti. (2017).
Poly(oligo(ethylene glycol) methyl ether methacrylate) brushes on high-κ metal oxide dielectric surfaces for bioelectrical environments. D.Youngjin Joh; F. McGuire; R. Abedini-Nassab; J.B. Andrews; R.K. Achar; Z. Zimmers; D. Mozhdehi; R. Blair; F. Albarghouthi; W. Oles; J. Richter; C.M. Fontes; A.M. Hucknall; B.B. Yellen; A.D. Franklin; A. Chilkoti. (2017).
Crystallization kinetics of binary colloidal monolayers. A.T. Pham; R. Seto; J. önke; D.Y. Joh; A. Chilkoti; E. Fried; B.B. Yellen. (2016).
Micro- and nanostructured poly[oligo(ethylene glycol)methacrylate] brushes grown from photopatterned halogen initiators by atom transfer radical polymerization. S.A. Ahmad; G.J. Leggett; A. Hucknall; A. Chilkoti. (2011).
Protein Patterning by UV-Induced Photodegradation of Poly(oligo(ethylene glycol) methacrylate) Brushes. S. Alang Ahmad; A. Hucknall; A. Chilkoti; G.J. Leggett. (2010).
Simple Fabrication of Antibody Microarrays on Nonfouling Polymer Brushes with Femtomolar Sensitivity for Protein Analytes in Serum and Blood. A. Hucknall; D.H. Kim; S. Rangarajan; R.T. Hill; W.M. Reichert; A. Chilkoti. (2009).
In Pursuit of Zero: Polymer Brushes that Resist the Adsorption of Proteins. A. Hucknall; S. Rangarajan; A. Chilkoti. (2009).
Versatile synthesis and micropatterning of nonfouling polymer brushes on the wafer scale. A. Hucknall; A.J. Simnick; R.T. Hill; A. Chilkoti; A. Garcia; M.S. Johannes; R.L. Clark; S. Zauscher; B.D. Ratner. (2009).
Protein-Resistant Polymer Coatings on Silicon Oxide by Surface-Initiated Atom Transfer Radical Polymerization. H. Ma; D. Li; X. Sheng; B. Zhao; A. Chilkoti. (2006).
“Non-Fouling” Oligo(ethylene glycol)- Functionalized Polymer Brushes Synthesized by Surface-Initiated Atom Transfer Radical Polymerization. H. Ma; J. Hyun; P. Stiller; A. Chilkoti. (2004).