Genetically Encoded Polypeptide for Affinity Capture and Purification of Biologics

TitleGenetically Encoded Polypeptide for Affinity Capture and Purification of Biologics
Publication TypePatent
Year of Publication2021
AuthorsLuginbuhl, KM, Chilkoti, A
Patent Version NumberUS20210340186A1
International Patent NumberUS20210340186A1
International Patent ClassificationA1
Application Number17/349558
Date Published11/04/2021
Patent Number17349558
Abstract

The invention relates to genetically encoded fusion proteins comprised of a capture component that binds a target with high affinity and a peptide polymer, such as elastin-like polypeptides, that display phase behavior and can be used for purification. The invention further relates to methods for optimizing capture fusion proteins for individual biologic targets such that phase separation occurs under desirable conditions, such as at room temperature, lower concentrations of salt, and/or at suitable pH ranges and optimized capture domains and polypeptides with phase behavior that have been identified by the optimization methods.