Publications

Yang, Zhongping, and Ashutosh Chilkoti. “Microstamping of a biological ligand onto an activated polymer surface.” Advanced Materials 12, no. 6 (2000): 413–17. https://doi.org/10.1002/(SICI)1521-4095(200003)12:63.0.CO;2.

Hoffman, Allan S., Patrick S. Stayton, Volga Bulmus, Guohua Chen, Jingping Chen, Chuck Cheung, Ashutosh Chilkoti, et al. “Really smart bioconjugates of smart polymers and receptor proteins.” Journal of Biomedical Materials Research 52, no. 4 (2000): 577–86. https://doi.org/10.1002/1097-4636(20001215)52:43.0.CO;2-5.

Frey, Wolfgang, and Ashutosh Chilkoti. “Ultraflat nanosphere lithography: functionalized nanostructures for biomedical applications.” Annals of Biomedical Engineering 28, no. SUPPL 1 (2000): 94.

Frey, W., C. K. Woods, and A. Chilkoti. “Ultraflat nanosphere lithography: a new method to fabricate flat nanostructures.” Adv. Mater. (Germany) 12, no. 20 (2000): 1515–19. https://doi.org/10.1002/1521-4095(200010)12:203.0.CO;2-J.

Yang, Zhongping, W. Frey, T. Oliver, and A. Chilkoti. “Light-activated affinity micropatterning of proteins on self-assembled monolayers on gold.” Langmuir (USA) 16, no. 4 (2000): 1751–58. https://doi.org/10.1021/la998079.

Meyer, D. E., G. Kong, M. Dewhirst, and A. Chilkoti. “Genetically-engineered polypeptide carrier for thermal targeting of therapeutics.” Annual International Conference of the IEEE Engineering in Medicine and Biology Proceedings 2 (December 1, 1999): 1289.

Stayton, P. S., S. Freitag, L. A. Klumb, A. Chilkoti, V. Chu, J. E. Penzotti, R. To, et al. “Streptavidin-biotin binding energetics.” Biomolecular Engineering 16, no. 1–4 (December 1999): 39–44. https://doi.org/10.1016/s1050-3862(99)00042-x.

Freitag, S., I. Le Trong, L. A. Klumb, V. Chu, A. Chilkoti, P. S. Stayton, and R. E. Stenkamp. “X-ray crystallographic studies of streptavidin mutants binding to biotin.” Biomolecular Engineering 16, no. 1–4 (December 1999): 13–19. https://doi.org/10.1016/s1050-3862(99)00048-0.

Piervincenzi, R. T., and A. Chilkoti. “Controlling the orientation of immobilized proteins by genetic circular permutation.” Annual International Conference of the IEEE Engineering in Medicine and Biology Proceedings 1 (December 1, 1999): 93.

Wong, J., A. Chilkoti, and V. T. Moy. “Direct force measurements of the streptavidin-biotin interaction.” Biomolecular Engineering 16, no. 1–4 (December 1999): 45–55. https://doi.org/10.1016/s1050-3862(99)00035-2.

Yang, Z. P., and A. Chilkoti. “Light-activated affinity micropatterning of proteins.” Annual International Conference of the IEEE Engineering in Medicine and Biology Proceedings 2 (December 1, 1999): 738.

Meyer, D. E., and A. Chilkoti. “Purification of recombinant proteins by fusion with thermally-responsive polypeptides.” Nat Biotechnol 17, no. 11 (November 1999): 1112–15. https://doi.org/10.1038/15100.

Meyer, D. E., and A. Chilkoti. “Polypeptide fusion tag for thermal purification of recombinant proteins.” ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 217 (March 21, 1999): U177–U177.

Freitag, S., I. Le Trong, A. Chilkoti, L. A. Klumb, P. S. Stayton, and R. E. Stenkamp. “Structural studies of binding site tryptophan mutants in the high-affinity streptavidin-biotin complex.” Journal of Molecular Biology 279, no. 1 (May 1998): 211–21. https://doi.org/10.1006/jmbi.1998.1735.

Hoffman, A. S., P. S. Stayton, T. Shimoboji, G. Chen, Z. Ding, A. Chilkoti, C. Long, et al. “Conjugates of Stimuli-Responsive Polymers and Biomolecules: Random and Site-Specific Conjugates of Temperature-Sensitive Polymers and Proteins.” Macromolecular Symposia 118 (January 1, 1997): 553–62. https://doi.org/10.1002/masy.19971180173.

Shimoboji, T., P. S. Stayton, C. Long, A. Chilkoti, G. Chen, J. M. Harris, and A. S. Hoffman. “Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein.” Transactions of the Annual Meeting of the Society for Biomaterials in Conjunction with the International Biomaterials Symposium 1 (December 1, 1996): 897.

Frey, W., W. R. Schief, D. W. Pack, C. T. Chen, A. Chilkoti, P. Stayton, V. Vogel, and F. H. Arnold. “Two-dimensional protein crystallization via metal-ion coordination by naturally occurring surface histidines.” Proceedings of the National Academy of Sciences of the United States of America 93, no. 10 (May 1996): 4937–41. https://doi.org/10.1073/pnas.93.10.4937.

Chilkoti, A., B. L. Schwartz, R. D. Smith, C. J. Long, and P. S. Stayton. “Engineered chimeric streptavidin tetramers as novel tools for bioseparations and drug delivery.” Bio/Technology (Nature Publishing Company) 13, no. 11 (November 1995): 1198–1204. https://doi.org/10.1038/nbt1195-1198.

Stayton, P. S., T. Shimoboji, C. Long, A. Chilkoti, G. Chen, J. M. Harris, and A. S. Hoffman. “Control of protein-ligand recognition using a stimuli-responsive polymer.” Nature 378, no. 6556 (November 1995): 472–74. https://doi.org/10.1038/378472a0.

Chilkoti, A., T. Boland, B. D. Ratner, and P. S. Stayton. “The relationship between ligand-binding thermodynamics and protein-ligand interaction forces measured by atomic force microscopy.” Biophysical Journal 69, no. 5 (November 1995): 2125–30. https://doi.org/10.1016/s0006-3495(95)80083-4.

Chilkoti, A., A. E. Schmierer, V. H. Pérez-Luna, and B. D. Ratner. “Investigating the relationship between surface chemistry and endothelial cell growth: partial least-squares regression of the static secondary ion mass spectra of oxygen-containing plasma-deposited films.” Analytical Chemistry 67, no. 17 (September 1995): 2883–91. https://doi.org/10.1021/ac00113a024.

Schwartz, B. L., J. E. Bruce, G. A. Anderson, S. A. Hofstadler, A. L. Rockwood, R. D. Smith, A. Chilkoti, and P. S. Stayton. “Dissociation of tetrameric ions of noncovalent streptavidin complexes formed by electrospray ionization.” Journal of the American Society for Mass Spectrometry 6, no. 6 (June 1995): 459–65. https://doi.org/10.1016/1044-0305(95)00191-f.

Chilkoti, A., P. H. Tan, and P. S. Stayton. “Site-directed mutagenesis studies of the high-affinity streptavidin-biotin complex: contributions of tryptophan residues 79, 108, and 120.” Proceedings of the National Academy of Sciences of the United States of America 92, no. 5 (February 1995): 1754–58. https://doi.org/10.1073/pnas.92.5.1754.

Schwartz, B. L., D. C. Gale, R. D. Smith, A. Chilkoti, and P. S. Stayton. “Investigation of non‐covalent ligand binding to the intact streptavidin tetramer by electrospray ionization mass spectrometry.” Journal of Mass Spectrometry 30, no. 8 (January 1, 1995): 1095–1102. https://doi.org/10.1002/jms.1190300806.

Chilkoti, A., and P. S. Stayton. “Molecular Origins of the Slow Streptavidin—Biotin Dissociation Kinetics.” Journal of the American Chemical Society 117, no. 43 (January 1, 1995): 10622–28. https://doi.org/10.1021/ja00148a003.